Main Characters[]
| Character | Voice actor | Appearances |
| Flash | ||
| Impulse | ||
| Jesse Quick | ||
| Kid Flash |
| Character | Voice actor | Appearances |
| Reverse-Flash | ||
| Gorilla Grodd | ||
| Black Flash | ||
| Speedwarp | ||
| Joanie Swift | ||
| Kid Zoom |
| Character | Voice actor | Appearances |
| Iris Allen | ||
| Jay Garrick | ||
| Joan Garrick | ||
| Rudolph West | ||
| Mary West | ||
| Linda Park | ||
| Iris West | ||
| Jai West | ||
| Owen Mercer | ||
| Dante Ramone |
Recurring characters[]
| Character | Voice actor | Appearances |
| Pollux | ||
| Lady Flash | ||
| XS | ||
| Green Lantern | ||
| Superman | ||
| The Atom | ||
| Black Canary | ||
| Red Tornado | ||
| Plastic Man | ||
| Hawkman | ||
| Nightwing | ||
| Hawkgirl | ||
| Cyborg | ||
| Jade | ||
| Power Girl | ||
| Arrowette | ||
| Vibe | ||
| Gypsy | ||
| Congorilla | ||
| Cosmic Geek | ||
| Kurt Lance | ||
| Turbocharger | ||
| Sprint |
| Character | Voice actor | Appearances |
| Captain Cold | ||
| Abra Kadabra | ||
| Lady Lightning | ||
| Shade | ||
| Sportsmaster | ||
| Savitar | ||
| Baroness Blitzkrieg | ||
| Magenta | ||
| Icicle | ||
| Mirror Master | ||
| Heat Wave | ||
| Turbine | ||
| Weather Wizard | ||
| Golden Glider | ||
| Trickster | ||
| Captain Boomerang | ||
| Mob Rule | ||
| Brainstorm | ||
| Neutron |
| Character | Voice actor | Appearances |
| Helen Claiborne | ||
| Darryl P. Frye | ||
| James Forrest | ||
| Chester P. Runk | ||
| Charles | ||
| Gregory Wolfe | ||
| Dexter Myles |
Minor characters[]
| Character | Voice actor | Appearances |
| The Flash II | ||
| Don Allen | ||
| Dawn Allen | ||
| Element Woman | ||
| Saint Walker | ||
| Lightray | ||
| Black Racer | ||
| Lightstar | ||
| Hot Spot | ||
| Peek-a-Boo | ||
| Pied Piper | ||
| Teryx | ||
| Dac |
| Character | Voice actor | Appearances |
| Steg |
| Character | Voice actor | Appearances |
| Nora Allen | ||
| Henry Allen | ||
| Meloni Thawne | ||
| Malcolm Allen | ||
| Valerie Perez | ||
| Kristen Kramer | ||
| Brendan Bowman | ||
| Fred Chyre | ||
| Jared Morillo | ||
| Clyde Mardon | ||
| Elsa Mardon | ||
| Josh Jackham |